EPA Method 8310 Polynuclear Aromatic Hydrocarbons - Revision 0
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C32BA02BB7424FD6992200D40AC44306 |
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文件大小(MB)： |
0.13 |
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页数： |
13 |
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日期： |
2003-1-16 |
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8310 1,CD-ROM Revision 0,Date September 1986,METHOD 8310,POLYNUCLEAR AROMATIC HYDROCARBONS,1.0 SCOPE AND APPLICATION,1.1 Method 8310 is used to determine the concentration of certain polynuclear,aromatic hydrocarbons (PAH) in ground water and wastes. Specifically,Method 8310 is used to detect the following substances:,Acenaphthene Chrysene,Acenaphthylene Dibenzo(a,h)anthracene,Anthracene Fluoranthene,Benzo(a)anthracene Fluorene,Benzo(a)pyrene Indeno(1,2,3-cd)pyrene,Benzo(b)fluoranthene Naphthalene,Benzo(ghi)perylene Phenanthrene,Benzo(k)fluoranthene Pyrene,1.2 Use of Method 8310 presupposes a high expectation of finding the,specific compounds of interest. If the user is attempting to screen samples for,any or all of the compounds listed above, he must develop independent protocols,for the verification of identity.,1.3 The method detection limits for each compound in reagent water are,listed in Table 1. Table 2 lists the practical quantitation limit (PQL) for,other matrices. The sensitivity of this method usually depends on the level of,interferences rather than instrumental limitations. The limits of detection,listed in Table 1 for the liquid chromatographic approach represent sensitivities,that can be achieved in the absence of interferences. When interferences are,present, the level of sensitivity will be lower.,1.4 This method is recommended for use only by experienced residue analysts,or under the close supervision of such qualified persons.,2.0 SUMMARY OF METHOD,2.1 Method 8310 provides high performance liquid chromatographic (HPLC),conditions for the detection of ppb levels of certain polynuclear aromatic,hydrocarbons. Prior to use of this method, appropriate sample extraction,techniques must be used. A 5- to 25-uL aliquot of the extract is injected into,an HPLC, and compounds in the effluent are detected by ultraviolet (UV) and,fluorescence detectors.,2.2 If interferences prevent proper detection of the analytes of interest,the method may also be performed on extracts that have undergone cleanup using,silica gel column cleanup (Method 3630).,8310 2,CD-ROM Revision 0,Date September 1986,TABLE 1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF PAHsa,Column Method Detection,Retention capacity limit (ug/L),Compound time (min) factor (k') UV Fluorescence,Naphthalene 16.6 12.2 1.8,Acenaphthylene 18.5 13.7 2.3,Acenaphthene 20.5 15.2 1.8,Fluorene 21.2 15.8 0.21,Phenanthrene 22.1 16.6 0.64,Anthracene 23.4 17.6 0.66,Fluoranthrene 24.5 18.5 0.21,Pyrene 25.4 19.1 0.27,Benzo(a)anthracene 28.5 21.6 0.013,Chrysene 29.3 22.2 0.15,Benzo(b)fluoranthene 31.6 24.0 0.018,Benzo(k)fluoranthene 32.9 25.1 0.017,Benzo(a)pyrene 33.9 25.9 0.023,Dibenzo(a,h)anthracene 35.7 27.4 0.030,Benzo(ghi)perylene 36.3 27.8 0.076,Indeno(1,2,3-cd)pyrene 37.4 28.7 0.043,a HPLC conditions: Reverse phase HC-ODS Sil-X, 5 micron particle size, in,a 250-mm x 2.6-mm I.D. stainless steel column. Isocratic elution for 5 min using,acetonitrile/water (4:6)(v/v), then linear gradient elution to 100% acetonitrile,over 25 min at 0.5 mL/min flow rate. If columns having other internal diameters,are used, the flow rate should be adjusted to maintain a linear velocity of 2,mm/sec.,TABLE 2. DETERMINATION OF PRACTICAL QUANTITATION LIMITS (PQL) FOR VARIOUS,MATRICESa,Matrix Factorb,Ground water 10,Low-level soil by sonication with GPC cleanup 670,High-level soil and sludges by sonication 10,000,Non-water miscible waste 100,000,aSample PQLs are highly matrix-dependent. The PQLs listed herein are,provided for guidance and may not always be achievable.,bPQL = [Method Detection Limit (Table 1) X [Factor (Table 2)]. For nonaqueous,samples, the factor is on a wet-weight basis.,8310 3,CD-ROM Revision 0,Date September 1986,3.0 INTERFERENCES,3.1 Solvents, reagents, glassware, and other sample processing hardware may,yield discrete artifacts and/or elevated baselines, causing misinterpretation of,the chromatograms. All of these materials must be demonstrated to be free from,interferences, under the conditions of the analysis, by running method blanks.,Specific selection of reagents and purification of solvents by distillation in,all-glass systems may be required.,3.2 Interferences coextracted from the samples will vary considerably from,source to source. Although a general cleanup technique is provided as part of,this method, individual samples may require additional cleanup approaches to,achieve the sensitivities stated in Table 1.,3.3 The chromatographic conditions described allow for a unique resolution,of the specific PAH compounds covered by this method. Other PAH compounds, in,addition to matrix artifacts, may interfere.,4.0 APPARATUS AND ……
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